Antimicrobial Formulation of a Bacterial Nanocellulose/Propolis-Containing Photosensitizer for Biomedical Applications

With the aim of contributing to the development of more efficient materials for wound care, new topical formulations based on bacterial nanocellulose (BNC) hydrogels containing propolis were produced. Characterizations confirmed the incorporation of propolis into the BNC matrix, maintaining its structure and properties. Rheological analysis confirmed that the hydrogels showed thixotropic behavior appropriate for topical application. Chromatographic profiles showed sustained release of propolis biomarkers for at least 20 h. The formulations did not present mutagenicity. For application in photodynamic inactivation (PDI), BNC/propolis hydrogels were prepared with the photosensitizers methylene blue (MB). Spectroscopy and confocal fluorescence microscopy confirmed the interaction of MB and propolis in BNC hydrogels, as well as the formation of a new composite material. In the antibacterial assays, formulations containing MB and propolis significantly reduced Staphylococcus aureus growth. In the presence of light, BNC/MB hydrogels completely inhibited the microorganism. Therefore, the results suggest potential materials for the prevention or treatment of Staphylococcus aureus infections in wounds.


Introduction
Wound healing is a complex and dynamic process influenced by local and systemic factors that involve reactions and interactions between cells, biomolecules, and mediators occurring in proper sequence and time [1]. The normal healing process is traditionally divided into the integrated and overlapped phases of inflammation, proliferation, and remodeling [2]. However, normal physiologic responses can be affected by many factors, which may impair progress and lead to delayed acute wounds and chronic wounds [3].
Infection is one of the main causes of delay or impediment in wound repair because it generates an intense immune response and tissue damage as a consequence of excessive microbial proliferation at the injury site. In addition, the bacterial biofilm present in most chronic wounds hinders the penetration of antimicrobials and contributes to antibiotic tolerance and resistance, which further complicate treatment [4,5]. Chronic wounds represent a significant clinical challenge for patients and healthcare professionals due to the Methylene blue (MB) is an organic dye derived from phenothiazine that has fluorescent and photosensitizing properties. Therefore, it is widely used in PDI as a photosensitizer. MB can eliminate malignant cells and inactivate viruses, fungi, and bacteria through photodynamic reactions [50,51].
In this work, we report the development and characterization of new BNC hydrogelbased formulations containing propolis and MB. The advantage, compared to conventional dressings (free-standing films or membranes), is that the hydrogel allows for better spreading and dispersion over the infected areas, in addition to enabling applications in more complex wounds that have tunnels, adhering the material throughout the targeted area (due to adequate rheological properties). In view of existing dressings reported in the literature based on BNC and propolis, this study presents the progress of several treatments, mainly with respect to the applicability of hydrogels based on BNC and in association with photodynamic therapy. From a hydrogel-based material, new properties can be obtained, such as high exudate capacity and non-adherence (easily removed from the wound), in addition to being easily developed and handled. Specifically, the material proposed in this work allows for the use of light to enhance treatment against microorganisms around wounds. All BNC hydrogels were fully characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and rheology. We also elaborated a drug delivery profile and evaluated in vitro antimicrobial activity, including confocal photodynamic inactivation against S. aureus.

Bacterial Nanocellulose Pulp
Wet BNC was obtained from static cultivation of the bacterium Komagataeibacter rhaeticus in Hestrin and Schramm (HS) liquid medium composed of 50 g L −1 glucose, 4 g L −1 yeast extract, 0.73 g L −1 magnesium sulfate (MgSO 4 ), 20 mL ethanol, and 980 mL distilled water. After 5 days of incubation at 28 • C, the membranes were treated in a 0.1 M sodium hydroxide (NaOH) solution to remove residues and washed in water until reaching neutral pH. The hydrated BNC membranes were ground in a high-speed dispersing element (Ultraturrax) under aqueous conditions. The suspension of BNC microparticles was sieved, resulting in a BNC pulp.

Characterization of Bacterial Nanocellulose Hydrogels Containing Propolis
A JEOL field-emission scanning electron microscope (JMF-6700F-Field Emission SEM/Analytical Field Emission SEM) was used to evaluate the morphology and microstructure of the BNC hydrogels and observe the influence of propolis on the formation of composites. For the analysis, freeze-dried samples were coated with a carbon layer and placed on copper supports. FTIR spectra were obtained to confirm the molecular interaction between propolis and the BNC matrix using Cary 630 FTIR equipment (Agilent Technologies, Santa Clara, CA, USA) in the range of 650 to 4000 cm −1 with 64 scans and a 4 cm −1 resolution. Measurements were performed with the freeze-dried formulations.
Thermogravimetric analysis was performed to evaluate the thermal behavior of BNC/propolis hydrogels using an SDT Q600 instrument (TA Instruments, New Castle, DE, USA). The freezedried samples were heated at a constant rate of 10 • C min −1 from 30 • C to 600 • C under a nitrogen flow of 100 mL min −1 .
Flow curves were determined by rheological assays conducted at room temperature (25 • C) using a TA Instruments rheometer (AR1500) equipped with a 2 • cone geometry. The shear rate ranged from 0 to 280 Pa s −1 for the upward flow for 120 s and from 280 to 0 Pa s −1 for the downward flow for 120 s. The results were analyzed by TRIOS software v 5.1.1.
Chemical characterization of propolis extract was carried out by high-performance liquid chromatography (HPLC) using a Shimadzu (Kyoto, Japan) liquid chromatograph equipped with a CBM-20A controller, an LC-20AT quaternary pump, an SPD-M 20A diodearray detector, and Lab Solution Software version 5.92. A Shimadzu Shim-Pack column (4.6 mm × 250 mm, particle diameter of 5 µm, and pore diameter of 100 Å) was used. The mobile phase consisted of methanol (B) and a solution of water-formic acid (0.1% v/v), pH 2.7 (A). A linear gradient of 20-95% of B was used for 77 min at a flow rate of 0.8 mL min −1 , with an injection volume of 10 µL. The column oven was set to 40 • C, and detection was set at 275 nm. The compounds were identified by comparison with chromatographic standards p-coumaric acid obtained from Sigma Aldrich (Saint Louis, MO, USA); artepillin C from Wako Chemical Industries Co. (Osaka, Japan); and baccharin isolated, purified, and supplied by Professor Jairo Kenupp Bastos from the University of São Paulo. For these analyses, the formulations were diluted in methanol (HPLC grade) and 0,1% formic acid (1.5:1). The samples were filtered through a 0.45 µL filter before injection.
Release studies were performed in vitro using a Franz diffusion cell at 37 • C. The receptor compartment was filled with phosphate buffer (pH 7.5), 0,9% sodium chloride (NaCl), and 12% hydrogenated ethoxylated castor oil (Cremophor RH40 OE). Then, 500 mg of the hydrogels was deposited in the donating compartment, and 500 µL of receptor solution samples was collected from the Franz cell at predetermined intervals, followed by replacement with the same volume of pure receptor solution. Samples were analyzed using the HPLC methodology to quantify p-coumaric acid and artepillin C, both of which are propolis biomarkers. Mathematical dilution adjustments were considered to correctly determine the content of the markers in the samples. The experiments were carried out in duplicate, and the propolis release profiles were determined. To evaluate the release kinetics of biomarkers, zero-order, first-order, and Higuchi mathematical models were applied.
Mutagenic potential analysis was accomplished according to the preincubation methodology developed by Maron and Ames (1983) [52] using TA98, TA100, TA102, and TA97a strains of Salmonella typhimurium in experiments with (+S9) and without (−S9) metabolic activation. The S9 microsomal fraction, a freeze-dried Sprague-Dawley rat liver homogenate, was purchased from Moltox Molecular Toxicology Inc. (Boone, NC, USA). Hydrogels were solubilized in dimethyl sulfoxide (DMSO), reaching the non-toxic concentration of 0.01 g mL −1 [53]. After defrosting, the bacterial stock culture was sown in Oxoid Nutrient Broth nº 2 and incubated for 18 h (overnight) at 37 • C, obtaining a density of 1-2 × 10 9 bacteria mL −1 . Then, 0.1 mL of this bacterial culture, 0.5 mL of 0.2 M phosphate buffer, or 0.5 mL of 4% S9 mixture was added to the samples, followed by incubation. Subsequently, 2 mL of top agar supplemented with histidine and biotin was added to the tubes and plated on minimal agar. After 48 h of incubation at 37 • C, the number of histidine-revertant colonies per plate (His + ) was counted manually. All tests were performed in triplicate. The standard mutagens used to confirm the reversion properties and specificity of each strain in experiments without the S9 mixture were 4-nitroo-phenylenediamine (10.0 µg/plate) for TA98 and TA97a, sodium azide (1.25 µg/plate) for TA100, and mitomycin C (0.5 µg/plate) for TA102. In the experiments with metabolic activation, 2-aminoanthracene (1.25 µg/plate) was used for TA98, TA97a, and TA100, and 2-aminofluorene (10.0 µg/plate) was used for TA102. DMSO was used as negative control (100 µL/plate). Data from the Ames test were expressed as mean ± standard deviation (SD), and the statistical significance was determined by one-way analysis of variance (ANOVA) complemented by Dunnett's test (post hoc test) to compare the results with those of the control group (DMSO). The mutagenic index (MI) was also calculated as the average number of revertants per test plate divided by the average number of revertants per solvent (negative control plate). A sample was considered mutagenic if the ANOVA variation was significant, with p < 0.05 and an average increase in revertants in the sample of a minimum of two folds of that found in the negative control (MI > 2) [54].

Characterization of Bacterial Nanocellulose Hydrogels Containing Propolis/Methylene Blue
Ultraviolet and visible (UV-Vis) absorption spectroscopy was performed to characterize the absorption profile of the BNC hydrogels containing the photosensitizer MB and the influence of propolis on its behavior. The scanning range was 350 to 800 nm. The hydrogels, in their natural form, were spread over glass slides (1 mm thick) and analyzed in a Varian Cary ® 50 Bio spectrophotometer.
For fluorescence analysis, a portable prototype fluorescence spectroscopy system was used, consisting of a spectrophotometer (USB2000, Ocean Optics-Palo Alto, CA, USA, a "Y" type fiber Ocean Optics), a light source, filters, and a computer. The excitation was provided by a 405 nm diode laser, and the fluorescence emission was acquired by the spectrophotometer, which was connected to a computer using OOIBase software (Ocean Optics, Orlando, FL, USA) [55].
To evaluate the morphology and spatial fluorescence characteristics of BNC/MB hydrogels and their behavior in the presence of propolis, a Zeiss-LSM780 inverted confocal fluorescence microscope was used. The samples were excited by a diode laser emitting at 405 nm with a power of 40 µW cm −2 and operating in continuous (cw) and pulsed mode. The pixel dwell was fixed at 30 µs, and fluorescence spectra were acquired in the range of 400-700 nm (resolution of 8 nm) by high-sensibility GaAsP photomultipliers. For the assay, the samples were previously freeze-dried and analyzed on glass slides [56].
The antimicrobial photodynamic activity of hydrogels was investigated against Staphylococcus aureus (ATCC 25923) using microdilution and the agar diffusion methods. The experimental groups were BNCH/P1, BNCH/P1/MB1, BNCH/P1/MB2, BNCH/MB1, and BNCH/MB2, and the control groups were BH and BNCH. The assays were performed with and without light irradiation at 660 nm.
Staphylococcus aureus was cultivated in tryptic soy broth (TBS). To this end, 7-8 bacterial colonies were inoculated in 10 mL of TSB and incubated at 37 • C overnight. Then, 500 µL of inoculum was transferred to 9.5 mL of fresh TBS, and this sample was incubated at 37 • C for 4 h to reach the mid-log phase. The turbidity of the bacterial suspension was adjusted in a spectrophotometer at 600 nm to obtain 10 8 colony-forming units per mL (CFU/mL).
For the microdilution method, the bacterial suspension was centrifugated at 3000 rpm for 15 min and resuspended in phosphate-buffered saline (PBS). Then, 0.15 g aliquots of each hydrogel were transferred into 24-well plates, followed by 400 µL aliquots of the bacterial suspension in PBS. After homogenization, the samples were left in the dark for 20 min at room temperature, then irradiated by red light at 660 nm with a light dose of 50 J cm −2 . Later, 100 µL aliquots of each sample were diluted in 900 µL of PBS, and five 10-fold serial dilutions were carried out for each sample. Finally, a volume of 15 µL from each dilution was streaked onto brain-heart infusion (BHI) agar plates according to the spread plate method. Plates were incubated at 37 • C for 24 h for the bacterial count. The agar diffusion method was also used to verify the antimicrobial photodynamic activity of hydrogels.
Based on the CLSI disk diffusion method, the freeze-dried hydrogels were cut into 1 cm 2 films. Then, 100 µL aliquots of the bacterial suspension were streaked onto BHI agar plates according to the spread plate method. After drying, the pieces of hydrogel films were placed in the center of each Petri dish quadrant. The plates from the experimental groups were irradiated at 660 nm with a light dose of 50 J cm −2 , and, in the end, all cultures, including the control groups that were not illuminated, were incubated at 37 • C for 24 h. The inhibition zone halo was measured with a ruler, and the values were noted in millimeters. All experimental antimicrobial studies were performed in triplicate.
Statistical analysis of the photodynamic inactivation results was performed using Origin software, and the data were evaluated by one-way analysis of variance (ANOVA). The differences between all experimental groups were evaluated by ANOVA, followed by Tukey's post hoc test, with the significance level set at p < 0.05.

Scanning Electron Microscopy (SEM)
Electron microscopic images of the formulations ( Figure 1A) show a large quantity of micro-and nanofibers in samples containing BNC (from II to V), in contrast to the control group (I), which presents an evidently homogenous aspect. The hydrogels containing propolis maintained the native BNC structure of a three-dimensional network formed by its nanofibers. The fibrils remain in a random arrangement with a high degree of interlacing, which confirms the classical porous structure of BNC [57]. In general, the diameter of nanofibers ranges from 20 to 100 nm [58]. Figure 1(AV) shows a more compact fiber structure compared to the other formulations, suggesting the effective incorporation of propolis into the biopolymer matrix. A higher concentration of propolis in the hydrogels results in a uniform filing of the BNC nanometric pores.

Fourier Transform Infrared Spectroscopy (FTIR)
According to the spectra ( Figure 1B), all samples present with a band around 3300 cm −1 , characteristic of OH stretching vibration, which was more intense in hydrogels containing BNC due to its greater water absorption. The bands at 1680 cm −1 and 1700 cm −1 correspond to the 2 C=O and C=C stretching of the flavonoids and aromatic rings from other phenolic compounds of propolis, respectively [59]. Both vibrations are present in the spectrum of pure EPP-AF ® and in the hydrogels that contain it, indicating the incorporation of propolis in the formulations, which corroborates the SEM results. Another typical band that confirms propolis in composite hydrogels occurs around 830 cm −1 , referring to the angular deformation of aromatic rings [60].
In the region of 2850 cm −1 , there is a discrete band characteristic of EPP-AF ® , which is repeated in hydrogels and becomes more pronounced in the formulation with the highest concentration of propolis (BNCH/P3). The bands between 2850 and 2970 cm −1 in the pristine BNC, BH, and solid EPP-AF ® spectra were superimposed on the hydrogels, indicating a possible interaction between the components and composite formation.

Thermogravimetric Analysis (TGA)
As shown in Figure 1C, BNC exhibits a typical thermal profile, which consists of two main events. In the first event, there is a small loss of mass (10%) in the temperature range of 30 to 150 • C, which is attributed to water loss. The second event occurs between 250 • C and 350 • C and results in a large mass loss (around 70-80%) related to the depolymerization of cellulose and the decomposition of glycosidic units, followed by the formation of carbonic residues [20,61]. BNC hydrogel (BNCH) presented a thermal profile similar to that of pristine BNC. However, the first degradation step occurs between 25 • C and 200 • C and comprises other mass loss events, probably due to the presence of pure hydrogel components (BH). Natrosol ® (hydroxyethyl cellulose) is an ester derived from cellulose, the thermal behavior of which is similar to that of BNC but with slightly lower thermal stability [62]. As shown in Figure 1C, BNC exhibits a typical thermal profile, which consists of two main events. In the first event, there is a small loss of mass (10%) in the temperature range of 30 to 150 °C, which is attributed to water loss. The second event occurs between 250 °C and 350 °C and results in a large mass loss (around 70-80%) related to the depolymerization of cellulose and the decomposition of glycosidic units, followed by the formation of carbonic residues [20,61]. BNC hydrogel (BNCH) presented a thermal profile similar to that of pristine BNC. However, the first degradation step occurs between 25 °C and 200 °C and comprises other mass loss events, probably due to the presence of pure hydrogel The EPP-AF ® curve shows associated events of propolis component decomposition, either successive and/or simultaneous, at room temperature with 5% loss. This event is related to the evaporation of volatile compounds present in the extract [63]. Much of the propolis degradation occurs between 200 • C and 500 • C, with mass loss of around 60% (at 275 • C), which is attributed to the carbon bond breakdown and degradation of organic compounds. BNC/propolis hydrogels presented intermediate thermal behavior between BNCH and EPP-AF ® , with a slight decrease in thermal stability. According to Barud et al. (2013) [20], the degradation of formulations starts only at high temperatures, enabling application to the skin. The hydrogels start to lose mass continuously at a maximum rate around 290 • C, ending at 550 • C. These events are related to the condensation of OH groups, the breaking of carbon bonds, and the degradation of organic compounds from propolis, as well as the degradation of BNC [63,64].
It is noteworthy that the TGA curve of the hydrogel with the highest concentration of propolis (BNCH/P3) is similar to the profile of pure propolis, while the thermal behavior of the hydrogel with the lowest proportion of the extract (BNCH/P1) corresponds to the mass loss events of BH and BNCH. These data corroborate the SEM and FTIR results, confirming the new composite formulation.

Rheological Analysis
Rheological flow properties are related to the deformation of the material when it is subjected to shear stress, providing information about its behavior and stability, which is fundamental for quality control, material acceptance, and effectiveness [65,66]. All formulations showed non-Newtonian behavior, since there was no linear proportional ratio between shear stress and shear rate. Figure 1D shows the flow curve of a representative sample of a BNC-based hydrogel. In terms of rheological behavior, the flow curve shows the pseudoplastic behavior that results from the alignment of the disrupted threedimensional network in the system in the direction of flow, providing the protective film formation characteristic that allows the skin surface to be covered, promoting improved protection [66].
The viscosity of hydrogels decreases with increasing shear rate. When this rate is removed, the hydrogels gradually recover their values through microrestructuring of formulation elements. Furthermore, an area of hysteresis is formed between the upward and downward flow curves, which characterizes thixotropic behavior [67]. This is an important characteristic for topical application formulations due to the ease of removing the contents of the package and allowing the product to be spread on the skin, preventing runoff [68,69]. Materials with thixotropic characteristics also tend to have increased shelf life, as they maintain constant viscosity during storage, avoiding the separation of constituents from the formulations [66].
In general, in the presence of BNC, there is an increase in complex viscosity compared to pure BH for all hydrogels. Specifically, for formulations containing propolis, the behavior is very similar between samples with different concentrations (BNCH/P1, BNCH/P2, and BNCH/P3). There is a slight gradual increase in viscosity associated with an increase in propolis contents ( Figure 2). Furthermore, there is an overlapping of curves with similar values for all samples, i.e., the rheological properties remain comparable with the same inclination values.

. Chemical Profile of EPP-AF ® Biomarkers and Release Study
A chromatographic analysis of biomarkers from EPP-AF ® was performed to confirm the composition of propolis extract according to the methodology previously published by Berretta et al. (2012) [24]. The obtained chemical profile (Figure 3) identifies characteristic peaks of (1) p-coumaric acid, (2) artepillin C, and (3) baccharin, the amounts of which are listed in Table 1.

Chemical Profile of EPP-AF ® Biomarkers and Release Study
A chromatographic analysis of biomarkers from EPP-AF ® was performed to confirm the composition of propolis extract according to the methodology previously published by Berretta et al. (2012) [24]. The obtained chemical profile (Figure 3) identifies characteristic peaks of (1) p-coumaric acid, (2) artepillin C, and (3) baccharin, the amounts of which are listed in Table 1. The results are comparable to the chemical profile of EPP-AF ® [24], confirming the proper incorporation of the extract into the formulations. As expected, the negative controls, BH and BNCH, do not present characteristic peaks of propolis.  The results are comparable to the chemical profile of EPP-AF ® [24], confirming the proper incorporation of the extract into the formulations. As expected, the negative controls, BH and BNCH, do not present characteristic peaks of propolis.
The amounts of p-coumaric acid and artepillin C identified in the hydrogels are evidently proportional to the concentration of propolis extract used in each formulation (BNCH/P1-1.2% EPP-AF ® , BNCH/P2-2.4% EPP-AF ® , and BNCH/P3-3.6% EPP-AF ® ). However, the proportion was not maintained for baccharin, possibly due to its degradation during the protocol, as well as lower stability or greater difficulty in extracting the compound. The release study was carried out with propolis hydrogels to evaluate its biomarker release over time (Figure 4).
Although artepillin C is present in the propolis extract in its highest concentration according to the chromatographic profile, it was released in lower proportions compared to p-coumaric acid. Similar results were obtained in BNC membranes containing EPP-AF ® microemulsion; the authors attributed this behavior to the polarity of the biomarkers [32]. Possibly due to its greater hydrophilicity, 50% of p-coumaric acid was released from all hydrogels in a period of 2 h to 3 h, while the same percentage of lipophilic artepillin C was released in an average time of 45 h among tested formulations.
(BNCH/P1-1.2% EPP-AF ® , BNCH/P2-2.4% EPP-AF ® , and BNCH/P3-3.6% EPP-A However, the proportion was not maintained for baccharin, possibly due to its degr tion during the protocol, as well as lower stability or greater difficulty in extracting compound. The release study was carried out with propolis hydrogels to evaluate it omarker release over time (Figure 4). Although artepillin C is present in the propolis extract in its highest concentra according to the chromatographic profile, it was released in lower proportions comp to p-coumaric acid. Similar results were obtained in BNC membranes containing EPPmicroemulsion; the authors attributed this behavior to the polarity of the biomarkers Possibly due to its greater hydrophilicity, 50% of p-coumaric acid was released from hydrogels in a period of 2 h to 3 h, while the same percentage of lipophilic artepill was released in an average time of 45 h among tested formulations.
In all hydrogels, artepillin C release was more gradual compared to that of p-cou ric acid. BNCH/P1 released both biomarkers faster than the other biomembranes, poss due to the lower propolis concentration and, consequently, reduced interaction betw the propolis compounds and BNC, favoring faster delivery. A higher content o omarkers in more concentrated formulations can generate more chemical interaction tween propolis molecules and the BNC matrix, such as hydrogen and Van der W bonds, trapping these compounds in the nanometric networks of BNC and hinderin release. This hypothesis corroborates MEV results, which show that the nanofibers denser and more compacted in more concentrated formulations. The slower release with an increase in propolis in the hydrogels, can be attributed to the greater numbe intermolecular bonds [70].
The maximum release of p-coumaric acid reaches 90-100% in 21 h, in contrast to for artepillin C in 72 h. Despite the difference between the biomarkers, both releases be considered prolonged compared to other studies using BNC as a drug delivery sys In all hydrogels, artepillin C release was more gradual compared to that of p-coumaric acid. BNCH/P1 released both biomarkers faster than the other biomembranes, possibly due to the lower propolis concentration and, consequently, reduced interaction between the propolis compounds and BNC, favoring faster delivery. A higher content of biomarkers in more concentrated formulations can generate more chemical interactions between propolis molecules and the BNC matrix, such as hydrogen and Van der Waals bonds, trapping these compounds in the nanometric networks of BNC and hindering its release. This hypothesis corroborates MEV results, which show that the nanofibers are denser and more compacted in more concentrated formulations. The slower release rate, with an increase in propolis in the hydrogels, can be attributed to the greater number of intermolecular bonds [70].
The maximum release of p-coumaric acid reaches 90-100% in 21 h, in contrast to 75% for artepillin C in 72 h. Despite the difference between the biomarkers, both releases can be considered prolonged compared to other studies using BNC as a drug delivery system. Fontes et al., (2018) [71] produced BNC/carboxymethylcellulose composites and obtained a 70-80% delivery burst in 15 min, a significantly shorter time than that obtained in this work. The result of a longer release is interesting, considering the topical application of the formulations. Once the action of hydrogels is extended, it is possible to promote gradual bacterial inhibition, which can facilitate treatment and wound healing [72].
The release kinetics of propolis biomarkers from BNC hydrogels were determined based on mathematical functions that describe the release profile through correlation coefficients (r). The values obtained for each formulation are shown in Table 2. According to the r values, it is possible to infer that both biomarkers follow the Higuchi model, which is characteristic of all BNC/propolis hydrogels. This profile suggests the diffusion type of biomarker release from the BNC matrix [73], which occurs mainly due to its insolubility [74].
3.1.6. Mutagenic Potential Table 3 shows the mean (M) and standard deviation (SD) of the number of revertants per plate and mutagenicity index (MI, values in parentheses) in TA 98, TA 100, TA97a, and TA 102 strains of S. typhimurium after treatment with hydrogels according to experiments without and with metabolic activation (−S9 and +S9, respectively).  All experiments presented MI values below 2.0, which indicates that the hydrogels did not induce a statistically significant increase in the number of revertants compared to the negative control (DMSO), both in the presence and absence of metabolic activation. BNCH/EPP3 generated more sensitivity to the used strains, as evidenced by the reduction in the number of revertants compared to the negative control and the decrease in MI values. Nevertheless, data demonstrate the absence of mutagenic activity of all formulations under the applied conditions. The non-mutagenic effect of propolis was confirmed in other works [53,75]. The positive control of each cell line produced the expected mutagenic response and was used to validate the susceptibility of the system to standard mutagens. Figure 5 presents the UV-Vis and fluorescence emission spectra for the formulations prepared for analysis of photodynamic inactivation. The spectra of the BH and BNCH base formulations ( Figure 5A) show no absorption band, which is expected due to the high transparency of the Natrosol ® hydrogel (BH) and the light scattering caused by the high content of cellulose nanofibers in the BNCH [76].

Spectroscopic Analysis
BNC hydrogels containing MB present two main absorption bands, with maxima centered around 670 nm and 610 nm, with the highest and the lowest intensities, respectively, in the less concentrated formulation (BNCH/MB1). However, in the most concentrated formulation (BNCH/MB2), these bands have their intensities inverted, suggesting an MB dimerization process ( Figure 6). According to the literature, MB is in balance between its monomeric and dimeric forms, which have maximum absorbance around 660 nm and 610 nm, respectively, similar to our results. At higher concentrations of MB, there is a tendency for dye aggregation, generating an equilibrium shift for the formation of dimers and band inversion [50]. In formulations containing MB and EPP-AF ® , it is possible to observe a slight shift of the bands from 670 and 610 nm to 680 and 620 nm, respectively, probably caused by the interaction between MB and propolis. Figure 5 presents the UV-Vis and fluorescence emission spectra for the formulations prepared for analysis of photodynamic inactivation. The spectra of the BH and BNCH base formulations ( Figure 5A) show no absorption band, which is expected due to the high transparency of the Natrosol ® hydrogel (BH) and the light scattering caused by the high content of cellulose nanofibers in the BNCH [76]. BNC hydrogels containing MB present two main absorption bands, with maxima centered around 670 nm and 610 nm, with the highest and the lowest intensities, respectively, in the less concentrated formulation (BNCH/MB1). However, in the most concentrated formulation (BNCH/MB2), these bands have their intensities inverted, suggesting an MB dimerization process ( Figure 6). According to the literature, MB is in balance between its monomeric and dimeric forms, which have maximum absorbance around 660 nm and 610 nm, respectively, similar to our results. At higher concentrations of MB, there is a tendency for dye aggregation, generating an equilibrium shift for the formation of dimers and band inversion [50]. In formulations containing MB and EPP-AF ® , it is possible to observe a slight shift of the bands from 670 and 610 nm to 680 and 620 nm, respectively, probably caused by the interaction between MB and propolis. The BNC/propolis hydrogel also does not present absorption bands the spectrum. Some previous studies on the ethanolic extract of propoli maximum absorbance around 295 nm, as well as absorption of phenolic der UV light [70,75]. However, this wavelength was not addressed in this PDI application uses visible light, in this case at 660 nm due to the MB ma ance.

Spectroscopic Analysis
In the fluorescence spectra of the formulations ( Figure 5B), there are sion bands: one between 450 nm and 550 nm and another between 650 n corresponding to blue/green and red regions, respectively. Except for B BNCH/MB2, all samples present an emission band around 500 nm. Only taining MB have a band at 700 nm, suggesting the emission of the dye, w state, emits fluorescence at 680 nm [77,78]. The BNC/propolis hydrogel also does not present absorption bands in this region of the spectrum. Some previous studies on the ethanolic extract of propolis have reported maximum absorbance around 295 nm, as well as absorption of phenolic compounds under UV light [70,75]. However, this wavelength was not addressed in this work since the PDI application uses visible light, in this case at 660 nm due to the MB maximum absorbance.
In the fluorescence spectra of the formulations ( Figure 5B), there are two main emission bands: one between 450 nm and 550 nm and another between 650 nm and 750 nm, corresponding to blue/green and red regions, respectively. Except for BNCH/MB1 and BNCH/MB2, all samples present an emission band around 500 nm. Only hydrogels containing MB have a band at 700 nm, suggesting the emission of the dye, which, in its pure state, emits fluorescence at 680 nm [77,78].
The band at 675 nm appears only in the spectrum of the BNCH/P1 formulation, which is assigned to emission from the propolis extract. In hydrogels containing EPP-AF ® and MB, the band at 675 nm appears more discrete, and bands around 700 nm are absent, which indicates a probable suppression of fluorescence due to the interaction of propolis with MB.

Confocal Fluorescence Microscopy
Images obtained by confocal fluorescence microscopy (Figure 7) are in accordance with the emission spectra described in the previous section. The formulations containing MB show fluorescence emission in red ( Figure 7B), corresponding to the bands around 700 nm observed in the spectra. The BNCH/P1 image ( Figure 7C) exposes the emission in the blue/green region, as well as the presence of some emission traces in the red region, which correspond to the bands around 500 and 675 nm, respectively. In the image of the formulation containing EPP-AF ® and MB ( Figure 7D), the emission in the blue region is predominant, which corroborates the high emission observed in the spectra of the BNCH/P1/MB1 and BNCH/P1/MB2 hydrogels around 500 nm. The images also corroborate the macroscopic aspect and the SEM images of the h drogels, which have porous spaces in their structures. Porous materials contribute to realization of PDT/PDI due to the need for the presence of oxygen for photodynamic actions to occur and because they favor the incorporation of assets in their matrices [79 Figure 8 and Table 4 show the results of the PDI treatment against S. aureus media by the MB present in BNC/propolis formulations. The BNC hydrogels containing propo and/or MB were tested to compare antimicrobial photodynamic activity of isolated M and MB in the presence of EPP-AF ® , both in hydrogel form. The images also corroborate the macroscopic aspect and the SEM images of the hydrogels, which have porous spaces in their structures. Porous materials contribute to the realization of PDT/PDI due to the need for the presence of oxygen for photodynamic reactions to occur and because they favor the incorporation of assets in their matrices [79].  Table 4 show the results of the PDI treatment against S. aureus mediated by the MB present in BNC/propolis formulations. The BNC hydrogels containing propolis and/or MB were tested to compare antimicrobial photodynamic activity of isolated MB and MB in the presence of EPP-AF ® , both in hydrogel form. BNCH/MB1 and BNCH/MB2 formulations showed a significant reduction in S. aureus growth (2.61 ± 0.01 and 3.55 ± 0.17 log CFU/mL, respectively), even in the experiments without light (p < 0.05). This result confirms the toxicity of MB in the dark [80]. However, in the absence of light, the antimicrobial activity of BNCH/MB2 was significantly higher than that of BNCH/MB1, demonstrating the dose-dependent action of MB. In the presence of light, both hydrogels containing MB completely eradicated the microorganism (p = 0), suggesting that these are the most suitable formulations for PDI against S. aureus.

Antimicrobial and Photodynamic Inactivation Assays
The microdilution results are in agreement with the disk diffusion experiment results (Table 4). It was possible to observe that BNC hydrogels containing only MB presented larger bacterial growth inhibition zones. The inhibition zone was especially significant in the most concentrated formulation (BNCH/MB2), the halo of which reached 5.0 ± 0.1 mm in the experiment with light irradiation, confirming the photodynamic effect of the formulations containing MB against S. aureus.
The photodynamic activity of MB occurs through type I reactions, which produce reactive oxygen species such as hydroxyl, and through type II reactions, which generate singlet oxygen. This species is considered the main cause of antimicrobial photodynamic activity, and the phenothiazine dye is well known for its high quantum yield of singlet oxygen [80,81]. Accordingly, MB-mediated PDI efficacy is well-founded and has been demonstrated in several studies and clinical applications, indicating a significant effect against a broad range of microorganisms, including resistant strains of S. aureus and P. aeruginosa [5].
Regarding the BNCH-and BH-based hydrogels, the formulation containing only propolis (BNCH/P1) significantly reduced bacterial growth (reduction of 2.15 log CFU/mL and inhibition zone of 3 ± 0.1 mm), but there was no significant difference between these formulations in the treatments with and without light (p > 0.05). This confirms that the antimicrobial activity of EPP-AF ® is not influenced by the presence of light [82,83]. In a similar work, BNC membranes containing 1.2% propolis, as well as the formulation tested in this experiment, were able to inhibit S. aureus growth, generating an inhibition zone of  BNCH/MB1 and BNCH/MB2 formulations showed a significant reduction in S. aureus growth (2.61 ± 0.01 and 3.55 ± 0.17 log CFU/mL, respectively), even in the experiments without light (p < 0.05). This result confirms the toxicity of MB in the dark [80]. However, in the absence of light, the antimicrobial activity of BNCH/MB2 was significantly higher than that of BNCH/MB1, demonstrating the dose-dependent action of MB. In the presence of light, both hydrogels containing MB completely eradicated the microorganism (p = 0), suggesting that these are the most suitable formulations for PDI against S. aureus.
The microdilution results are in agreement with the disk diffusion experiment results (Table 4). It was possible to observe that BNC hydrogels containing only MB presented larger bacterial growth inhibition zones. The inhibition zone was especially significant in the most concentrated formulation (BNCH/MB2), the halo of which reached 5.0 ± 0.1 mm in the experiment with light irradiation, confirming the photodynamic effect of the formulations containing MB against S. aureus.
The photodynamic activity of MB occurs through type I reactions, which produce reactive oxygen species such as hydroxyl, and through type II reactions, which generate singlet oxygen. This species is considered the main cause of antimicrobial photodynamic activity, and the phenothiazine dye is well known for its high quantum yield of singlet oxygen [80,81]. Accordingly, MB-mediated PDI efficacy is well-founded and has been demonstrated in several studies and clinical applications, indicating a significant effect against a broad range of microorganisms, including resistant strains of S. aureus and P. aeruginosa [5].
Regarding the BNCH-and BH-based hydrogels, the formulation containing only propolis (BNCH/P1) significantly reduced bacterial growth (reduction of 2.15 log CFU/mL and inhibition zone of 3 ± 0.1 mm), but there was no significant difference between these formulations in the treatments with and without light (p > 0.05). This confirms that the antimicrobial activity of EPP-AF ® is not influenced by the presence of light [82,83]. In a similar work, BNC membranes containing 1.2% propolis, as well as the formulation tested in this experiment, were able to inhibit S. aureus growth, generating an inhibition zone of 8 ± 0.58 mm [20]. The antibacterial activity of propolis is mainly related to the action of flavonoids and cinnamic acid derivatives present in its composition. Different bacterial inhibition values are due to differences in compounds and their contents in different extracts [83].
Hydrogels containing MB associated with propolis (BNCH/P1/MB1 and BNCH/P1/MB2) had significantly reduced PDI efficacy (p < 0.05) compared to those containing only MB, maintaining values close to 2 log CFU/mL of reduction for both in the dark. After light irradiation, the reduction in microbial growth increased to 4.30 log CFU/mL. Despite the favorable results of these formulations for PDI application, they suggest that propolis decreased the photodynamic action of MB against the bacteria. This may be related to the shift in MB maximum absorbance in the presence of propolis, as demonstrated by the UV-Vis spectra, which may have reduced the formation of reactive species responsible for the inactivation of the microorganism. Unlike the results obtained in this work, Wang et al. (2017) [84] achieved an increased phototoxicity of PS protoporphyrin IX (PpIX) by combining it with propolis. The synergistic effect between them significantly decreased tumor volume and mass in a xenographic model compared to PS alone.
In addition to the absorbance shift, there is the possibility of a reaction between MB, which has a positive charge [51], and the hydroxyls of the phenolic compounds of propolis, which have a negative charge [85], causing the inactivation of some active substances. However, it is possible that propolis reacted with 1 O 2 , acting as a sequestering agent and protecting bacteria from photodynamic effects.
The most accepted idea by the authors of this work regarding the reduction in the antimicrobial activity of BNCH/P/MB in relation to BNCH/MB concerns the turbidity of EPP-AF ® at the concentrations used. This may have hampered light transmission through formulations containing propolis, decreasing the amount of energy supplied to the PS, thereby reducing the formation of reactive species. The difficulty in performing antimicrobial tests for propolis has been reported in other works due to its turbidity [24]. However, to confirm this hypothesis, further tests with different concentrations of propolis are needed.
Nevertheless, all formulations containing EPP-AF ® and/or MB showed antimicrobial activity against S. aureus and may be possible future alternatives for wound care, since they may prevent or heal infections. The eradication of the microorganism by hydrogels containing MB after exposure to light expands the application of BNC formulations to PDI, supporting wound healing by eliminating infections.

Conclusions
New formulations of BNC hydrogels containing EPP-AF ® and MB photosensitizer were developed. The formulations maintained the BNC structure of a three-dimensional network of nanofibers, as well as its molecular organization and thermal stability. Furthermore, they presented pseudoplastic thixotropic behavior, making them suitable for topical applications. The quantity of propolis biomarkers in the formulations correspond to the concentration of the EPP-AF ® extract used, and their release was sustained for at least 20 h, representing a possible extended-release system. The obtained hydrogels did not present mutagenicity. In order to apply the formulations to photodynamic inactivation, BNC/propolis hydrogels containing MB were produced. Optical characterization showed maxima centered at 670 nm and 610 nm for pure MB formulations. In the most concentrated formulation, these bands have their intensities inverted, suggesting an MB dimerization process. There was a slight shift in the maximum absorbance of the dye with propolis to 680 nm and 620 nm, which may have led to a decrease in the PDI efficacy. Nevertheless, all formulations containing propolis and/or MB were able to reduce S. aureus growth on some scale. As an advantage, the BNC/MB hydrogels completely inhibited the growth of the bacteria after exposure to light, demonstrating great efficacy for application in PDI. We conclude that the new formulation of BNC produced in this study can potentially support wound healing by treating or preventing infections.

Data Availability Statement:
The data presented in this study are available on request from the corresponding authors.